Bulletin of the SFI 3 (148) 1999 - A study of polymorphism within growth hormone gene 2 in sea trout from Polish coastal rivers using heteroduplex analysis

A study of polymorphism within growth hormone gene 2 in sea trout from Polish coastal rivers using heteroduplex analysis
Bulletin of the Sea Fisheries Institute 3 (148) 1999, pp. 57-61

Ewa WŁODARCZYK, Anita Butowska and Roman Wenne
Sea Fisheries Institute, Kołłątaja 1, 81-332 Gdynia, Poland

Key words: sea trout, heteroduplex, growth hormone gene, heterozygosity, population genetics.

Abstract.
Polymorphism within growth hormone gene 2 (GH2), which is 1,489 bp in length and includes both introns and exons, was investigated in populations of sea trout from six Polish coastal rivers. The GH 2 fragment was amplified by PCR, and digested with the restriction enzyme HinfI. It was then analyzed using the heteroduplex method. Two alleles were identified and no significant differentiation in their frequency among the investigated populations was observed. Differentiation in the frequency of the heterozygotes was found. No deviation from the Hardy-Weinberg rule was observed in the investigated populations; this reveals that no mixing of genetically isolated populations occurs (the Wahlund effect).